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COPN restores mitochondrial quality control and interrupts the ROS‒cGAS‒inflammation axis in OGD/R-treated R28 cells. a , TEM images showing changes in the mitochondrial ultrastructure of R28 cells subjected to various treatments (PBS, PDNs, COPN-L, and COPN-H) after OGD/R. Scale bar: 500 nm b , Quantitative analyses of mitochondrial length and number in R28 cells as indicated in a . c , Representative fluorescence images showing JC-1 staining of the mitochondrial Δψm: aggregates (red) indicate healthy mitochondria, whereas monomers (green) represent depolarized mitochondria. Nuclei were stained with DAPI (blue). Scale bar: 50 μm d , Intracellular ATP content assay revealing improved energy production after COPN treatment under OGD/R stress conditions. e , mRNA expression analysis of mitochondrial dynamic regulatory genes <t>(Opa1,</t> Mfn2, Drp1, and Fis1) and mitochondrial DNA transcription levels (mt-ND1 and mt-COX1). f , Quantitative analysis of the JC-1 fluorescence ratio (aggregates/monomers) and mitochondrial ROS levels (MitoSOX staining). g–h , Western blot analysis of proteins involved in mitochondrial autophagy (Pink1, Parkin, and P62) (g) and mitochondrial fusion/fission (Opa1, Mfn2, Mfn1, Drp1, and Fis1) (h) . i , Confocal fluorescence microscopy images of mitochondria (green, MitoTracker) and lysosomes (red, LysoTracker) in R28 cells after different treatments. Nuclei were stained with Hoechst (blue). Scale bar: 50 μm ∗ ∗∗∗P < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Opa1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 412 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+opa1/OPA1+Rabbit+mAb/pmc13049686-160-30-31
Average 96 stars, based on 412 article reviews

opa1 - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "A dual-responsive CO-releasing nanogel ameliorates retinal ischemia–reperfusion injury by restoring mitochondrial homeostasis and attenuating cGAS-STING pathway activation"

Article Title: A dual-responsive CO-releasing nanogel ameliorates retinal ischemia–reperfusion injury by restoring mitochondrial homeostasis and attenuating cGAS-STING pathway activation

Journal: Materials Today Bio

doi: 10.1016/j.mtbio.2026.102974

Figure Legend Snippet: COPN restores mitochondrial quality control and interrupts the ROS‒cGAS‒inflammation axis in OGD/R-treated R28 cells. a , TEM images showing changes in the mitochondrial ultrastructure of R28 cells subjected to various treatments (PBS, PDNs, COPN-L, and COPN-H) after OGD/R. Scale bar: 500 nm b , Quantitative analyses of mitochondrial length and number in R28 cells as indicated in a . c , Representative fluorescence images showing JC-1 staining of the mitochondrial Δψm: aggregates (red) indicate healthy mitochondria, whereas monomers (green) represent depolarized mitochondria. Nuclei were stained with DAPI (blue). Scale bar: 50 μm d , Intracellular ATP content assay revealing improved energy production after COPN treatment under OGD/R stress conditions. e , mRNA expression analysis of mitochondrial dynamic regulatory genes (Opa1, Mfn2, Drp1, and Fis1) and mitochondrial DNA transcription levels (mt-ND1 and mt-COX1). f , Quantitative analysis of the JC-1 fluorescence ratio (aggregates/monomers) and mitochondrial ROS levels (MitoSOX staining). g–h , Western blot analysis of proteins involved in mitochondrial autophagy (Pink1, Parkin, and P62) (g) and mitochondrial fusion/fission (Opa1, Mfn2, Mfn1, Drp1, and Fis1) (h) . i , Confocal fluorescence microscopy images of mitochondria (green, MitoTracker) and lysosomes (red, LysoTracker) in R28 cells after different treatments. Nuclei were stained with Hoechst (blue). Scale bar: 50 μm ∗ ∗∗∗P < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Techniques Used: Control, Fluorescence, Staining, Expressing, Western Blot, Microscopy

other:

Article Title: Vesicle-mediated mitochondrial clearance presents an actionable metabolic vulnerability in triple-negative breast cancer.
Article Snippet: Antibodies include anti-PINK1 (Cell Signaling, 6946T), anti-Parkin-1 (Cell Signaling, 4211T), anti-NDP52 (Cell Signaling, 60732T), antiOPTN (Cell Signaling, 58981), anti-SQSTM1 (Cell Signaling, 8025T), anti-BNIP3L/Nix (Cell Signaling, 12396T), anti-LC3B (Cell Signaling, 368T), anti-DRP1 (Abcam, ab56788), anti-phospho-DRP1 (Ser616) (Cell Signaling, #4494), anti-phospho-DRP1 (Ser637) (Cell Signaling, #6319), anti-DMN2 (Santa Cruz, Sc-166669), anti-MFN1 (Cell Signaling, 14739S), anti-MFN2 (Cell Signaling, 9482S), anti-OPA1 (Cell Signaling, 67589S), anti-GAPDH (Santa Cruz, Sc-32233), and anti-Beta-Actin (Santa Cruz, Sc-47778).

Article Title: The second mitochondrial activator of caspases (SMAC) regulates growth, inflammation and mitochondrial integrity in cancer cells.
Article Snippet: Antibodies The following primary antibodies were used: anti-γH2AX (#9718S, Cell Signaling), anti-GAPDH (MAB374, Millipore), anti-cleaved caspase-3 (#9661, Cell Signaling Technology), anti-caspase-3 (#9662, Cell Signaling Technology), anti-SMAC (#2954 and #15108, Cell Signaling Technology), anticytochrome c (#11940 and #12963, Cell Signaling Technology), antiphospho-STING (#19781S, Cell Signaling Technology), anti-phospho-IRF3 (#ab76493, Abcam), anti-TFAM (#8076S, Cell Signaling Technology), antidsDNA (#ab27156, Abcam) anti-biotin (#SAB4200680-100UL, SigmaAldrich) anti-LAMP2 (#49067T, Cell Signaling Technology), anti-BiP (#3177T, Cell Signaling Technology), anti-COX IV-Alexa Flour 488 conjugate A Lung adenocarcinoma Colon-rectum adenocarcinmoa Cell Death & Differentiation (#4853S, Cell Signaling Technology), anti-OPA1 (#67589S, Cell Signaling Technology), anti MIC10 (#31561-1-AP, Proteintech) and anti-TOM20 (#42406, Cell Signaling Technology).

Article Title: Synergistic Neurotoxicity of environmental Cadmium and Paraquat in Parkinsonism: Unveiling the Mito-ROS/OPA1/Caspase-3/GSDME-driven Apoptosis Axis
Article Snippet: Anti-caspase-3 (9662, 1:1000), anti-cleaved-caspase-3 (9661, 1:1000), anti-caspase-9 (9502S, 1:1000), anti-cleaved-PARP (5625T, 1:1000), anti-OMA1 (95473S, 1:1000), anti-OPA1 (80471S, 1:1000), anti-MFN1 (14739, 1:1000), anti-MFN2 (11925, 1:1000), anti-pS616 DRP1 (4494, 1:1000), anti-pS637 DRP1 (6319, 1:1000) and anti-GSDMD (39754S, 1:1000) were procured from Cell Signaling Technology.

Article Title: Vesicle-mediated mitochondrial clearance presents an actionable metabolic vulnerability in triple-negative breast cancer
Article Snippet: anti-OPA1 , Cell Signaling , Cat. 67589S; RRID: AB_2799728.

Article Title: Protective role of fatty acid oxidation against epithelial barrier dysfunction in allergic asthma
Article Snippet: Primary antibodies used were anti-CPT1A (Abcam, UK, ab234111), anti-DRP1 (Cell Signaling Technology, CST, USA, 8570), anti-FIS1 (CST, 32525), anti-OPA1 (CST, 80471), anti-MFN2 (CST, 9482), anti-NLRP3 (CST, 15101), Anti-Caspase-1 (Abcam, ab179515), anti-p-AMPK (CST, 50081), anti-CD36 (CST, 14347), anti-GLUT (CST, 12939), anti-LDHA (CST, 2012), anti-ZO-1 (Abcam, ab276131), anti-occludin (Abcam, ab216327), and anti-β-actin (CST, 3700) at a dilution ratio of 1:1000.

Article Title: Vesicle-mediated mitochondrial clearance presents an actionable metabolic vulnerability in triple-negative breast cancer
Article Snippet: Antibodies include anti-PINK1 (Cell Signaling, 6946T), anti-Parkin-1 (Cell Signaling, 4211T), anti-NDP52 (Cell Signaling, 60732T), anti-OPTN (Cell Signaling, 58981), anti-SQSTM1 (Cell Signaling, 8025T), anti-BNIP3L/Nix (Cell Signaling, 12396T), anti-LC3B (Cell Signaling, 368T), anti-DRP1 (Abcam, ab56788), anti-phospho-DRP1 (Ser616) (Cell Signaling, #4494), anti-phospho-DRP1 (Ser637) (Cell Signaling, #6319), anti-DMN2 (Santa Cruz, Sc-166669), anti-MFN1 (Cell Signaling, 14739S), anti-MFN2 (Cell Signaling, 9482S), anti-OPA1 (Cell Signaling, 67589S), anti-GAPDH (Santa Cruz, Sc-32233), and anti-Beta-Actin (Santa Cruz, Sc-47778).

Incubation:

Article Title: HUWE1 regulates mitophagy to protect dopaminergic neurons from 6-OHDA- and MPP⁺-induced neurotoxicity.
Article Snippet: .. The membranes were blocked with 5% non-fat milk for 1 h at room temperature and incubated overnight at 4 °C with the following primary antibodies: anti-HUWE1 (1:1,000, #9482, Cell Signaling Technology, MA, USA), anti-TOMM20 (1:1,000, #42406, CST), anti-β-actin (1:1,000, sc-47778, Santa Cruz, CA, USA), anti-GAPDH (1:1,000, sc-32233, Santa Cruz), anti-Myc (1:1,000, #9B11, CST), anti-COX IV (1:1,000, #3E11, CST), anti-LC3B (1:1,000, #2775, CST), anti-Bcl-2 (1:1,000, sc-783, Santa Cruz), anti-Bax (1:1,000, sc-6236, Santa Cruz), anti-Cleaved caspase-3 (1:1,000, #9664, CST), anti-DRP1 (1:1,000, #8570, CST), anti-MFN2 (1:1,000, #9482, CST), anti-OPA1 (1:1,000, #80471, CST), anti-p-ULK1 (1:1,000, #5869, CST), anti-ULK1 (1:1,000, A8529, ABclonal, MA, USA), anti-AMBRA1 (1:1,000, A1083, ABclonal), and antiUbiquitin (1:1,000, #39269, CST). ..

Article Title: HUWE1 regulates mitophagy to protect dopaminergic neurons from 6-OHDA- and MPP⁺-induced neurotoxicity
Article Snippet: .. The membranes were blocked with 5% non-fat milk for 1 h at room temperature and incubated overnight at 4 °C with the following primary antibodies: anti-HUWE1 (1:1,000, #9482, Cell Signaling Technology, MA, USA), anti-TOMM20 (1:1,000, #42,406, CST), anti-β-actin (1:1,000, sc-47778, Santa Cruz, CA, USA), anti-GAPDH (1:1,000, sc-32233, Santa Cruz), anti-Myc (1:1,000, #9B11, CST), anti-COX IV (1:1,000, #3E11, CST), anti-LC3B (1:1,000, #2775, CST), anti-Bcl-2 (1:1,000, sc-783, Santa Cruz), anti-Bax (1:1,000, sc-6236, Santa Cruz), anti-Cleaved caspase-3 (1:1,000, #9664, CST), anti-DRP1 (1:1,000, #8570, CST), anti-MFN2 (1:1,000, #9482, CST), anti-OPA1 (1:1,000, #80,471, CST), anti-p-ULK1 (1:1,000, #5869, CST), anti-ULK1 (1:1,000, A8529, ABclonal, MA, USA), anti-AMBRA1 (1:1,000, A1083, ABclonal), and anti-Ubiquitin (1:1,000, #39269, CST). .. After washing, the membranes were incubated with HRP-conjugated secondary antibodies (anti-mouse or anti-rabbit, 1:5,000, Santa Cruz) for 1 h at room temperature.

Image Search Results

Rhy enhances mitochondrial function by promoting mitochondrial fusion. (A) Representative fluorescent images of mitochondria stained with Mito-tracker Red in PC12 cells. (B) Quantitative analysis of mitochondrial networks in PC12 cells using the MiNA plugin for ImageJ ( n = 5 images). (C) Mitochondrial morphology in primary midbrain neurons visualized by Mito-tracker Red. (D) Mitochondrial network parameters quantified by MiNA analysis in primary midbrain neurons ( n = 5 images). (E) High-resolution imaging of mitochondrial inner membranes using PKmito Orange (PKMO) microscopy. (F) Representative transmission electron microscopy (TEM) images of mitochondrial ultrastructure. (G) Mitochondrial membrane potential quantification by JC-1 ratio (Red/Green) in PC12 cells and primary midbrain neurons, n = 3 independent experiments. (H) Cellular ATP levels determined using ATPlite™ luminescence assay ( n = 3). (I) Western blotting analysis of DRP1, p-DRP1 Ser616 , p-DRP1 Ser637 , MFN2 and OPA1 levels, following 6-OHDA (150 μmol/L) and Rhy treatment (1, 2, 5 μmol/L), n = 3 independent experiments. The data are presented as mean ± SD. Multiple group comparisons were evaluated through one-way analysis of variance (ANOVA). P < 0.05 was considered statistically significant.

Journal: Acta Pharmaceutica Sinica. B

Article Title: Rhynchophylline rewires DLAT lipoylation via conformational control to reverse mitochondrial bioenergetic collapse against dopaminergic neuronal injury

doi: 10.1016/j.apsb.2026.06.016

Figure Lengend Snippet: Rhy enhances mitochondrial function by promoting mitochondrial fusion. (A) Representative fluorescent images of mitochondria stained with Mito-tracker Red in PC12 cells. (B) Quantitative analysis of mitochondrial networks in PC12 cells using the MiNA plugin for ImageJ ( n = 5 images). (C) Mitochondrial morphology in primary midbrain neurons visualized by Mito-tracker Red. (D) Mitochondrial network parameters quantified by MiNA analysis in primary midbrain neurons ( n = 5 images). (E) High-resolution imaging of mitochondrial inner membranes using PKmito Orange (PKMO) microscopy. (F) Representative transmission electron microscopy (TEM) images of mitochondrial ultrastructure. (G) Mitochondrial membrane potential quantification by JC-1 ratio (Red/Green) in PC12 cells and primary midbrain neurons, n = 3 independent experiments. (H) Cellular ATP levels determined using ATPlite™ luminescence assay ( n = 3). (I) Western blotting analysis of DRP1, p-DRP1 Ser616 , p-DRP1 Ser637 , MFN2 and OPA1 levels, following 6-OHDA (150 μmol/L) and Rhy treatment (1, 2, 5 μmol/L), n = 3 independent experiments. The data are presented as mean ± SD. Multiple group comparisons were evaluated through one-way analysis of variance (ANOVA). P < 0.05 was considered statistically significant.

Article Snippet: Membrane were blocked with 5% non-fat milk in TBST (1 h, room temperature), and then incubated with primary antibodies (overnight, 4 °C) against DLAT (Proteintech, 13426-1-AP); DRP1 (Biodragon, RM8248); p-DRP1 Ser616 (Biodragon, BD-PP1318); p-DRP1 Ser637 (Biodragon, BD-PP0841); OPA1 (MCE, HY- P80255 ); MFN2 (Biodragon, BD-PT2740); GAPDH (Proteintech, 60004-1-Ig); TH (Santa Cruz, sc-14007); Lipoic acid (Santa Cruz, sc-101354); VMAT2 (Santa Cruz, sc-390,285); HA (Solarbio, K200003M), SIRT2 (Abways, CY5865); SIRT4(Proteintech, 66543-1-Ig); LIAS (Proteintech, 11577-1-AP); AMPK α (Biodragon, RM1159); p-AMPK α (Biodragon, RM0088); p-STAT3 (Abmart, TC52210); p-EGFR (Abmart, PC1644); KIF5B(Abclonal, A20928); KIF5C(Biodragon, BD-PN3289); KIF1B(Biodragon, BD-PN4193).

Techniques: Staining, Imaging, Microscopy, Transmission Assay, Electron Microscopy, Membrane, Luminescence Assay, Western Blot

DLAT mediates Rhy-induced mitochondrial fusion regulation. (A) Experimental workflow for identifying potential targets of Rhy using thermal proteome profiling (TPP) strategy. (B) Volcano plot of TPP results and screening strategy for Rhy's anti-PD targets. (C) Schematic representation of the target screening and prioritization pipeline. (D) Western blotting analysis showing Rhy-regulated mitochondrial dynamics proteins (OPA1, p-DRP1 Ser637 ) in control versus DLAT-KO cells ( n = 3). (E, F) Representative confocal images (E) and quantification (F) of mitochondrial networks visualized by Mito-tracker Red ( n = 3 fields). (G) Super-resolution microscopy images of mitochondrial cristae structures labeled with PKmito Orange (PKMO), n = 3 images. (H, I) TMRE staining reveals mitochondrial membrane potential alterations ( n = 5 fields). The data are presented as mean ± SD. Multiple group comparisons were evaluated through one-way analysis of variance (ANOVA). P < 0.05 was considered statistically significant.

Journal: Acta Pharmaceutica Sinica. B

Article Title: Rhynchophylline rewires DLAT lipoylation via conformational control to reverse mitochondrial bioenergetic collapse against dopaminergic neuronal injury

doi: 10.1016/j.apsb.2026.06.016

Figure Lengend Snippet: DLAT mediates Rhy-induced mitochondrial fusion regulation. (A) Experimental workflow for identifying potential targets of Rhy using thermal proteome profiling (TPP) strategy. (B) Volcano plot of TPP results and screening strategy for Rhy's anti-PD targets. (C) Schematic representation of the target screening and prioritization pipeline. (D) Western blotting analysis showing Rhy-regulated mitochondrial dynamics proteins (OPA1, p-DRP1 Ser637 ) in control versus DLAT-KO cells ( n = 3). (E, F) Representative confocal images (E) and quantification (F) of mitochondrial networks visualized by Mito-tracker Red ( n = 3 fields). (G) Super-resolution microscopy images of mitochondrial cristae structures labeled with PKmito Orange (PKMO), n = 3 images. (H, I) TMRE staining reveals mitochondrial membrane potential alterations ( n = 5 fields). The data are presented as mean ± SD. Multiple group comparisons were evaluated through one-way analysis of variance (ANOVA). P < 0.05 was considered statistically significant.

Techniques: Western Blot, Control, Super-Resolution Microscopy, Labeling, Staining, Membrane

Rhy ameliorates mitochondrial dysfunction via suppression of the DLAT–SIRT4 axis in PD mice. (A) Representative transmission electron microscope images illustrating mitochondrial ultrastructure in substantia nigra neurons. (B, C) Protein levels of mitochondrial dynamics markers (DRP1, p-DRP1 Ser616 , p-DRP1 Ser637 , MFN2, OPA1) in SN tissues were analyzed by Western blotting ( n = 3). (D, E) Immunofluorescence co-staining demonstrating reduced DLAT–SIRT4 colocalization in substantia nigra following Rhy treatment ( n = 3). The data are presented as mean ± SD. Multiple group comparisons were evaluated through one-way analysis of variance (ANOVA). P < 0.05 was considered statistically significant.

Journal: Acta Pharmaceutica Sinica. B

Article Title: Rhynchophylline rewires DLAT lipoylation via conformational control to reverse mitochondrial bioenergetic collapse against dopaminergic neuronal injury

doi: 10.1016/j.apsb.2026.06.016

Figure Lengend Snippet: Rhy ameliorates mitochondrial dysfunction via suppression of the DLAT–SIRT4 axis in PD mice. (A) Representative transmission electron microscope images illustrating mitochondrial ultrastructure in substantia nigra neurons. (B, C) Protein levels of mitochondrial dynamics markers (DRP1, p-DRP1 Ser616 , p-DRP1 Ser637 , MFN2, OPA1) in SN tissues were analyzed by Western blotting ( n = 3). (D, E) Immunofluorescence co-staining demonstrating reduced DLAT–SIRT4 colocalization in substantia nigra following Rhy treatment ( n = 3). The data are presented as mean ± SD. Multiple group comparisons were evaluated through one-way analysis of variance (ANOVA). P < 0.05 was considered statistically significant.

Techniques: Transmission Assay, Microscopy, Western Blot, Immunofluorescence, Staining

Journal: Materials Today Bio

Article Title: A dual-responsive CO-releasing nanogel ameliorates retinal ischemia–reperfusion injury by restoring mitochondrial homeostasis and attenuating cGAS-STING pathway activation

doi: 10.1016/j.mtbio.2026.102974

Figure Lengend Snippet: COPN restores mitochondrial quality control and interrupts the ROS‒cGAS‒inflammation axis in OGD/R-treated R28 cells. a , TEM images showing changes in the mitochondrial ultrastructure of R28 cells subjected to various treatments (PBS, PDNs, COPN-L, and COPN-H) after OGD/R. Scale bar: 500 nm b , Quantitative analyses of mitochondrial length and number in R28 cells as indicated in a . c , Representative fluorescence images showing JC-1 staining of the mitochondrial Δψm: aggregates (red) indicate healthy mitochondria, whereas monomers (green) represent depolarized mitochondria. Nuclei were stained with DAPI (blue). Scale bar: 50 μm d , Intracellular ATP content assay revealing improved energy production after COPN treatment under OGD/R stress conditions. e , mRNA expression analysis of mitochondrial dynamic regulatory genes (Opa1, Mfn2, Drp1, and Fis1) and mitochondrial DNA transcription levels (mt-ND1 and mt-COX1). f , Quantitative analysis of the JC-1 fluorescence ratio (aggregates/monomers) and mitochondrial ROS levels (MitoSOX staining). g–h , Western blot analysis of proteins involved in mitochondrial autophagy (Pink1, Parkin, and P62) (g) and mitochondrial fusion/fission (Opa1, Mfn2, Mfn1, Drp1, and Fis1) (h) . i , Confocal fluorescence microscopy images of mitochondria (green, MitoTracker) and lysosomes (red, LysoTracker) in R28 cells after different treatments. Nuclei were stained with Hoechst (blue). Scale bar: 50 μm ∗ ∗∗∗P < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The following primary antibodies were used: Drp1 (CST, #8570, 1:1000, ∼80 kDa), Fis1 (Proteintech, 10956-1-AP, 1:1000, ∼17 kDa), Mfn1 (Abcam, ab104274, 1:1000, ∼84 kDa), Mfn2 (CST, #9482, 1:1000, ∼86 kDa), Opa1 (CST, #80471, 1:1000, ∼100–120 kDa), Pink1 (CST, #6946, 1:1000, ∼63 kDa), cGAS (CST, #15102, 1:1000, ∼60 kDa), STING (CST, #13647, 1:1000, ∼42–45 kDa), TBK1 (CST, #3013, 1:1000, ∼84 kDa), p-TBK1 (CST, #5483, 1:1000, ∼84 kDa), COX IV (Abcam, ab14744, 1:2000, ∼17 kDa), β-actin (Proteintech, 66009-1-Ig, 1:5000, ∼43 kDa), and GAPDH (Proteintech, 60004-1-Ig, 1:5000, After washing, the membranes were incubated with HRP-conjugated secondary antibodies (goat anti-rabbit or goat anti-mouse IgG, CST, 1:5000) for 1 h at room temperature.

Techniques: Control, Fluorescence, Staining, Expressing, Western Blot, Microscopy

Review

opa1 (Cell Signaling Technology Inc)

Structured Review

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1) Product Images from "A dual-responsive CO-releasing nanogel ameliorates retinal ischemia–reperfusion injury by restoring mitochondrial homeostasis and attenuating cGAS-STING pathway activation"

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